ngs图书馆准备套件

Illumina®和离子洪流Sequencing

NGS Library Prep Kits for Illumina®和离子洪流Sequencing

High-quality library preparation is a critical step in next-generation sequencing (NGS). PerkinElmer provides a complete portfolio of NGS library preparation kits and barcodes designed to increase the sensitivity, flexibility, and speed of library prep for the Illumina®and Ion Torrent排序平台。我们对我们的下一代测序库预备套件的提高致力于提高酶促效率,以满足客户需要每次实现稳健,可重复,高质量的测序结果的成本效益的解决方案。

Completely automated versions of our library prep kits are available for high throughput needs while manual versions cater to the needs of lower throughput users. To meet the demands of high-throughput customers, NEXTFLEX®试剂也可以以批量数和自定义格式提供。

/////我们的库预备套件被优化,以简化以下应用程序:

  • NGS Adapters|Barcoded adapters for all of your NGS multiplexing needs

  • Small RNA-Seq|可以通过小型RNA-SEQ研究基因表达的基因沉默和转录后调节,其允许样品中存在的所有小RNA和miRNA的分析

  • RNA-Seq|Profiling for the analysis of the expression level of RNAs in a given cell or population of cells

  • DNA-Seq|用于整个基因组测序和套件的图书馆预备试剂盒设计用于安捷伦或罗氏靶向诱因诱饵

  • Targeted Sequencing|针对高度有针对性的方法的针对性重新排序,分析特定的基因组区域,例如扩增子面板和完整的混合捕获解决方案

  • 16S and 18S rRNA Sequencing|16S and 18S ITS rRNA sequencing used to identify and compare bacteria or fungi present within an environmental sample.

  • Whole Genome Amplification|从单细胞和其他有限DNA模板中扩增DNA,用于NGS和其他应用

  • Preimplantation Genetic Testing|非倍性检测预体胚胎胚胎

Advancing Agricultural Genomics with Low-Cost NGS Genotyping

图书馆准备套件系列

NextFlex小RNA-SEQ与UDIS

Enhanced Data Security with UDIs to Propel your Small RNA Sequencing Applications


NEXTFLEX®独特的双重指数条形码适配器(UDIs) for sequencing on Illumina®platforms provide enhanced data security by drastically increasing the multiplexing capacity while reducing costs by allowing users to pool multiple libraries onto a single flow cell lane, all while mitigating index hopping. TheNEXTFLEX®带有UDIS的小型RNA-SEQ V3自动化套件利用流行的随机适配器技术融合了数据质量,完整性和准确性的新维度,用于减少小型RNA连接偏差,为PerkinElmer工作站提供完整和自动化的解决方案。最多192个UDI条形码引物可用于希望自信地增加Illumina的复用容量的用户®MiSeq®HiSeq®2000/2500和Novaseq®小型RNA测序应用的平台。

Rapid DNA-Seq XP

Illumina的自动DNA库准备用于测序®Platforms


NEXTFLEX®rapid XP DNA-seq kitcombines enzymatic fragmentation with end-repair and A-tailing in one reaction to create a highly efficient first step in library generation. Resulting DNA-seq libraries show consistent library size, high yield, low GC-bias, and high coverage. All required reagents for fragmentation, library prep, and magnetic bead-based cleanup is included in the kit, which should be bundled with theNEXTFLEX®条码选择。该DNA-SEQ Library Prep Kit可容纳从1 ng到1μg的样本输入范围,以为Illumina产生高质量的库®sequencing. Pre-plated reagents for automation are now available with single-useUnique Dual Index barcodes(UDIS)对于PerkinElmer工作站上的更简单的采用体验。

RHS Technology: DOPlify® Kit

使用正确的DNA扩增开始您的抗血入遗传测试


基因组分析平台需要至少1ng DNA,单细胞含有6 pg基因组DNA。全基因组扩增is used to replicate the complete genome to generate sufficient DNA for analysis. The accurate interpretation of single cell data relies on the ability of the whole genome amplification to comprehensively and reliably amplify the genome. TheDOPlify®kit已经专门针对单细胞全基因组扩增进行了优化。该试剂盒不仅适用于单细胞,而是对任何低模板样品,包括无细胞DNA和少量细胞。

Contact an NGS Expert

仅供研究使用。不用于诊断过程。